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氣管肺泡灌洗液 ( bronchoalveolar lavage fluid, BALF)流式檢測分群圈門策略

更新時間:2024-09-09   點擊次數(shù):106次

Carbapenem-resistant Klebsiella pneumoniae (CRKP)感染小鼠模型,氣管肺泡灌洗液 ( bronchoalveolar lavage fluid, BALF)流式檢測分群圈門策略,可以參考下圖。單核巨噬清除訂購信息見后。

氣管肺泡灌洗液 ( bronchoalveolar lavage fluid, BALF)流式檢測分群圈門策略

Gating strategy to characterize immune cells recovered in BALF. BALF cells were gated for live cells by excluding LIVE/DEAD fixable aqua-stained cells.

Leukocytes were gated as CD45+cells, which were further separated into Siglec-F+, CD11b+ and CD11b– cells. Siglec-F+ (SF+) cells were further gated into F4/80+ and CD11c+; both F4/80+ and CD11c+ cells were identified as alveolar macrophages (AMs). From CD11b+ cells, Ly6G+ cells were gated as neutrophils. CD11b+ Ly6G– cells were gated for interstitial macrophages (IMs) (CD11b+ F4/80+). Remaining cell populations were separated into Ly6Chi monocytes (CD11b+ Ly6Chi) and Ly6Clo monocytes (CD11b+ Ly6Clo). CD11b– cells were gated into B cells (B220+ cells) and remaining CD11b– B220–cells were separated into CD4+ T cells, CD8+ T cells, CD4+ CD8+ T cells and CD4– CD8– T cells(non-T & -B cells).



品牌:Liposoma

產(chǎn)地:荷蘭

貨號:CP-005-005

規(guī)格:5ml+5ml

品名:Clodronateliposomes & Control Liposomes

代理:靶點科技

部位:肺臟

細胞:肺泡巨噬細胞(AM)

劑量:70ul

方式:滴鼻(intratracheal administration

時間點:建模前48h

題目Lung-innervating nociceptor sensory neurons promote pneumonic sepsis during carbapenem-resistant Klebsiella pneumoniae lung infection

期刊:SCIENCE ADVANCES

時間:6 Sep 2024

期卷:Vol 10, Issue 36

鏈接:DOI: 10.1126/sciadv.adl6162


Liposoma巨噬細胞清除劑氯膦酸二鈉脂質(zhì)體清除肺泡巨噬細胞AM:

氣管肺泡灌洗液 ( bronchoalveolar lavage fluid, BALF)流式檢測分群圈門策略


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